background: accurate identification of sheep nematodes is a critical point in epidemiological studies and monitoring of drug resistance in flocks. however, due to a close morphological similarity between the eggs and larval stages of many of these nematodes,such identification is not a trivial task. there are a number of studies showing that molecular targets in ribosomal dna (internal transcri...

during spring and summer of 2010 and 2012, several surveys were conducted in different vineyards in kohgiluyeh & boyer-ahmad (southwestern iran) and kerman (southeastern iran) provinces, with the aim of isolating and identifying fungi associated with grapevine decline diseases. the samples collected comprised diseased grapevines showing yellowing, defoliation, reduced growth, wilting, intervein...

a specimen of an ascomycetous fungus collected during a field trip was identified as gymnoascus baran. soil samples collected from 0-20 cm depth were studied for isolation of fungi using a soil dilution plate method. based on the morphological and molecular characters the specimens were identified as gymnoascus reesii. genomic dna was extracted and a nuclear rdna region, containing the internal...

during 2004-2007, a field survey was conducted in different vineyards to determine the fungal pathogens associated with grapevine decline in different provinces of iran including fars,hamedan,isfahan, and kohgiluyeh and boirahmad. twenty-seven isolates of botryosphaeriaceae were recovered from vines showing decline and dieback symptoms only in fars province. based on morphological and cultural ...

Nuclear rDNA Internal Transcribed Spacers, ITS1 and ITS2, are widely used for eukaryote phylogenetic studies from the ordinal level to the species level, and there is even a database for ITS2 sequences. However, ITS regions have been ignored in mammalian phylogenetic studies, and only a few rodent and ape sequences are represented in GenBank. The reasons for this dearth, and the remedies, are d...

The current investigations suggest the segregation of Jurinea cartilaginea from Jurinea, and its placement among Centaureinae. Molecular studies, based on the nuclear ribosomal internal transcribed spacers (nrITS), chromosome number data and pollen morphology, support the morphological results. Based on these results, J. cartilaginea is related to and, therefore, transferred to the genus Karvan...

Sequences of ribosomal internal transcribed spacers (ITSs) are of great importance to molecular phylogenetics and DNA barcoding, but remain unstudied in some large taxa of Deuterostomia. We have analyzed complete ITS1 and ITS2 sequences in 62 species from 16 Deuterostomia classes, with ITS sequences in 24 species from 11 classes initially obtained using unannotated contigs and raw read sequence...

The DNA sequence of the intragenic region of the rat 45S ribosomal RNA precursor was determined. This sequence contains 2282 nucleotides and extends from the conserved EcoR I site near the 3' terminus of 18S rRNA to 69 nucleotides downstream of the 5' terminus of 28S rRNA. The sequences corresponding to 18S and 5.8S rRNA were identified by comparison with previously published data. The 5' termi...